The Possible Ameliorating Effect of Lactoferrin as adjuvant to Ceftriaxone on Induced Intestinal Sepsis in adult male albino rats (Histological and Immunohistochemical Study)

Document Type : Original Article

Authors

1 Demonstrator of histology and cell biology Faculty of Medicine - Benha University

2 Professor of Histology and Cell Biology Faculty of Medicine - Benha University

3 Asst. Professor of Histology and Cell Biology Faculty of Medicine - Benha University

Abstract

Background: Intra-abdominal infections can be seriously complicated to abdominal sepsis causing injury of various organs in the abdomen. Lactoferrin could be utilized in combination with Ceftriaxone to treat intestinal sepsis. Objective: to assess the possibility of Lactoferrin to ameliorate induced intestinal sepsis in adult male albino rats when used in combination with Ceftriaxone. Materials and methods: Fifty male rats were utilized in this study. The rats were divided into five groups: Group I (Control), Group II (Abdominal Sepsis model group): Rats received a single intraperitoneal injection of 1ml fresh fecal suspension (FSI) at a 50 mg/ml concentration. Group III (Abdominal Sepsis + Ceftriaxone group): Rats were treated with an intramuscular injection of Ceftriaxone, Group IV (Abdominal Sepsis + Lactoferrin group): Rats were treated with oral Lactoferrin, Group V (Abdominal Sepsis + Ceftriaxone + Lactoferrin group): Rats were treated with a combination of both. Colon samples were obtained and processed for histological examination. Results: Group II exhibited epithelial injury, detachment, and shedding; some crypts were damaged, obliterated, and lost parallelism; there was obvious depletion of goblet cells; and the lamina propria was markedly infiltrated with inflammatory cells. Furthermore, a significant increase in TNF-α, COX-2, and Caspase-3 immunoexpression was detected. Groups III and IV exhibited some histological attenuation, while group V showed histological architecture with more improvement. Conclusion:

Ceftriaxone can treat intestinal sepsis, but Lactoferrin can give better results when combined with Ceftriaxone.

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