Study of the Relationship Between Sirtuin 1 Rs7069102 Polymorphism and Diabetic Nephropathy in Egyptian Patients with Type 2 Diabetes Mellitus

Document Type : Original Article

Authors

1 Assistant Professor of Clinical and Chemical Pathology Faculty of Medicine - Benha Universiy

2 Consultant of Clinical and Chemical Pathology Faculty of Medicine - Benha University

3 Professor of Clinical and Chemical Pathology Faculty of Medicine - Benha University

4 Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt

5 Assistant Professor of Internal Medicine Faculty of Medicine - Benha University

Abstract

Background: Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia resulting from defects of insulin action, insulin secretion or both, causing macrovascular and microvascular complications, which can affect kidneys causing diabetic nephropathy (DN) which is one of the most feared diabetic chronic microvascular complications. Sirtuin 1 (SIRT1) is a NAD+-dependent deacetylase, belongs to a group of enzymes called silent information regulators. The aim of this work was to study the association between Sirtuin 1 rs7069102 polymorphism and diabetic nephropathy in Egyptian patients with T2DM.

Methods: This cross-sectional study was carried on 85 patients, who had T2DM for at least 10 years for assessment of the relationship between SIRT1 rs7069102 polymorphism and DN in patients with T2DM. Patients were subdivided into two groups: group I: included (45 patients) with T2DM and DN, and group II: included (40 patients) without DN. We analyzed the rs 7069102 polymorphism using StepOne real-time polymerase chain reaction(PCR) System and TaqMan SNP Genotyping assay.

Results: The CC genotype showed a higher percentage in patients with DN compared to those without DN (42.2 vs 7.5%). The percentage of CG genotype was significantly higher in patients without DN compared to those with DN (52.5% vs 46.7%, p=0.005), and the GG genotype was also significantly more prevalent in patients without DN (40.0% vs 11.1%, p

Keywords

Benha Medical Journal
Volume 42, Issue 9
Special issue (clinical and chemical pathology)
September 2025
Pages 72-84

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