Plasma level of MiR- 200b in type 2 diabetic retinopathy in Egyptian patients

Document Type : Original Article

Authors

1 Professor of Clinical and Chemical Pathology - Immunology Faculty of Medicine - Benha University

2 Assistant professor of Clinical and Chemical pathology Faculty of Medicine - Benha University

3 Assistant professor of Ophthalmology Faculty of Medicine - Benha University

4 Internal Medicine Fellowship Trainee Benha Health Insurance Hospital

5 department of of Clinical and Chemical pathology Faculty of Medicine - Benha University

6 Lecturer of Clinical and Chemical Pathology, Faculty of Medicine, Benha University

Abstract

Background: The incidence of diabetes mellitus is rapidly increasing, and it frequently causes considerable metabolic disorders and serious consequences. MicroRNA-200b (miR-200b) is a regulator of angiogenesis that has emerged as a diagnostic and predictive tool for certain disorders. Aim: to investigate the association of miR-200b with diabetic retinopathy (DR). Methods: The study included 100 subjects from Benha University Hospital: 40 patients with type 2 diabetes (T2D) without DR, 40 with T2D and DR, and 20 healthy controls. MicroRNA-200b was assessed for all subjects using real time PCR with SYBR Green. Results: Diabetics had lower levels of miR-200b than healthy controls. While, those without DR had even lower miR-200b levels compared to those who did have DR. Additionally, lower miR-200b levels were associated with neuropathy but not with nephropathy. Conclusion: MiR-200b showed promise as a biomarker for both T2D and DR. It perfectly differentiated diabetic patients with and without retinopathy, while offering moderate accuracy for diagnosing diabetes in general. These findings suggest miR-200b may play a risky role in DR among diabetics, and further research is warranted to confirm these results.

Keywords

Benha Medical Journal
Volume 41, Issue 8
December 2024
Pages 585-595

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