H .Shalan, N., Morgan, N., M.Abdrabh, R., M. Elbalshy, A., M. Abdalla, R. (2024). Significance of Transmembrane 9 Superfamily 4 (TM9SF4) immunohistochemical expression and nuclear morphometry in premalignant colonic lesions and colorectal carcinoma. Benha Medical Journal, 41(6), 60-72. doi: 10.21608/bmfj.2024.267320.2010
Naglaa H .Shalan; Norhan Mosaad Morgan; Rasha M.Abdrabh; Abd ElLatif M. Elbalshy; Rana M. Abdalla. "Significance of Transmembrane 9 Superfamily 4 (TM9SF4) immunohistochemical expression and nuclear morphometry in premalignant colonic lesions and colorectal carcinoma". Benha Medical Journal, 41, 6, 2024, 60-72. doi: 10.21608/bmfj.2024.267320.2010
H .Shalan, N., Morgan, N., M.Abdrabh, R., M. Elbalshy, A., M. Abdalla, R. (2024). 'Significance of Transmembrane 9 Superfamily 4 (TM9SF4) immunohistochemical expression and nuclear morphometry in premalignant colonic lesions and colorectal carcinoma', Benha Medical Journal, 41(6), pp. 60-72. doi: 10.21608/bmfj.2024.267320.2010
H .Shalan, N., Morgan, N., M.Abdrabh, R., M. Elbalshy, A., M. Abdalla, R. Significance of Transmembrane 9 Superfamily 4 (TM9SF4) immunohistochemical expression and nuclear morphometry in premalignant colonic lesions and colorectal carcinoma. Benha Medical Journal, 2024; 41(6): 60-72. doi: 10.21608/bmfj.2024.267320.2010
Significance of Transmembrane 9 Superfamily 4 (TM9SF4) immunohistochemical expression and nuclear morphometry in premalignant colonic lesions and colorectal carcinoma
1Department of anatomic Pathology, Faculty of medicine, Benha University
2M.B.B.CH anatomic Pathology, Faculty of medicine, Benha University
Abstract
Background: Although different markers are used as diagnostic methods for CRC such as; carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9), low sensitivity and low specificity of these markers revealed the importance of searching for other markers. Transmembrane 9 Superfamily 4 (TM9SF4) is autophagic protein involved in multiple stages of tumorigenesis and development. Nuclear morphometry is a method for digital histological analysis which has been subjected to significant technological advances in the last years. Aim: To evaluate Transmembrane 9 Superfamily 4 (TM9SF4) IHC expression and nuclear morphometry in premalignant colonic lesions and colorectal carcinoma. Material and methods: This is a retrospective study carried on 23 colorectal adenocarcinomas, 16 colonic adenomas, 11 ulcerative colitis and 6 normal colon cases as a control. Clinicopathological characteristics of examined cases were correlated with IHC of TM9SF4 and nuclear morphometry. Results: There is a significant statistical correlation between TM9SF4 scoring and different types of studied cases, lymphovascular invasion, lymph node metastasis, distant metastasis and stage of CRC. There is a significant statistical correlation between CRC and premalignant cases regarding nuclear morphometry. Also, there is a significant statistical correlation between area and perimeter nuclear morphometry and perineural invasion and grade of colorectal adenocarcinoma respectively. Conclusion: The results suggested that TM9SF4 has role in tumorigenesis and CRC development and also has a prognostic role in CRC while, nuclear morphometry has a diagnostic role with nuclear morphometry being better than TM9SF4.
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