Metwally, W., K. Helal, O., A.Morsy, S., E.Ahmed, N. (2024). The Possible Therapeutic Effect of Adipose Derived Mesenchymal Stem Cells and N-Acetyl cysteine on Bleomycin Induced Lung Fibrosis in Adult Male Albino Rats: Histological and Immuno-histochemical Study. Benha Medical Journal, 41(6), 17-29. doi: 10.21608/bmfj.2024.225748.1867
Wafaa Maged Metwally; Omayma K. Helal; Sherifa A.Morsy; Nessrine E.Ahmed. "The Possible Therapeutic Effect of Adipose Derived Mesenchymal Stem Cells and N-Acetyl cysteine on Bleomycin Induced Lung Fibrosis in Adult Male Albino Rats: Histological and Immuno-histochemical Study". Benha Medical Journal, 41, 6, 2024, 17-29. doi: 10.21608/bmfj.2024.225748.1867
Metwally, W., K. Helal, O., A.Morsy, S., E.Ahmed, N. (2024). 'The Possible Therapeutic Effect of Adipose Derived Mesenchymal Stem Cells and N-Acetyl cysteine on Bleomycin Induced Lung Fibrosis in Adult Male Albino Rats: Histological and Immuno-histochemical Study', Benha Medical Journal, 41(6), pp. 17-29. doi: 10.21608/bmfj.2024.225748.1867
Metwally, W., K. Helal, O., A.Morsy, S., E.Ahmed, N. The Possible Therapeutic Effect of Adipose Derived Mesenchymal Stem Cells and N-Acetyl cysteine on Bleomycin Induced Lung Fibrosis in Adult Male Albino Rats: Histological and Immuno-histochemical Study. Benha Medical Journal, 2024; 41(6): 17-29. doi: 10.21608/bmfj.2024.225748.1867
The Possible Therapeutic Effect of Adipose Derived Mesenchymal Stem Cells and N-Acetyl cysteine on Bleomycin Induced Lung Fibrosis in Adult Male Albino Rats: Histological and Immuno-histochemical Study
1Department of Histology and Cell Biology Benha Faculty of Medicine
2Professor of Histology and Cell Biology Benha Faculty of Medicine
3Professor of Histology and Cell Biology Benha Faculty of Medicine.
4Ass.Professor of histology and cell biology Benha Faculty of Medicine
Abstract
Background: Pulmonary fibrosis (PF) is a chronic fibrotic lung disease. Lung parenchyma and architecture is destroyed, compliance is lost, and gas exchange is compromised in this debilitating condition that leads to respiratory failure. NAC is a precursor of L-cysteine that results in glutathione elevation biosynthesis. It acts directly as a scavenger of free radicals, especially oxygen radicals. This study aimed to evaluate the possible therapeutic effect of Adipose derived mesenchymal stem cells and N-acetylcysteine (NAC) on induced lung fibrosis in adult male albino rats. Methods: Ten rats were used to isolated adipose-derived stem cells and sixty rats were randomly divided into five groups. Group I (control group: 20 rats), Group II (affected group:10 rats), Group III (Adipose stem cell group: 10 rats), Group Iv (N-acetylcystiene group:10 rats) and Group v (Adipose stem cell + NAC group: 10 rats). Specimens taken and histological studies, immunohistochemical and morphometric study were performed. Results: Group II showed markedly thickened inter-alveolar septa and destruction with extensive cellular infiltrations, increases in macrophage and alveoli were Collapsed.And there is massive lymphocytic infiltration around bronchiolar wall.There was a significant decrease (P0.01) in Ki67 expression in group III, IV & V compared to group I. Conclusion: Each of NAC and adipose tissue-derived stem cells can protect against lung fibrosis caused by Bleomycin when administered prior to, and concomitant with bleomycin, but their combined administration have better results.
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