Shaheen, N., Mousa, H. (2023). Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study. Benha Medical Journal, 40(Special issue (Academic)), 17-34. doi: 10.21608/bmfj.2022.153141.1630
Nehal Fahmy Shaheen; Hend Ragab Mousa. "Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study". Benha Medical Journal, 40, Special issue (Academic), 2023, 17-34. doi: 10.21608/bmfj.2022.153141.1630
Shaheen, N., Mousa, H. (2023). 'Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study', Benha Medical Journal, 40(Special issue (Academic)), pp. 17-34. doi: 10.21608/bmfj.2022.153141.1630
Shaheen, N., Mousa, H. Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study. Benha Medical Journal, 2023; 40(Special issue (Academic)): 17-34. doi: 10.21608/bmfj.2022.153141.1630
Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study
1Anatomy and Embryology Department ,Benha faculty of medicine, Benha university
2Anatomy and Embryology Department ,Benha faculty of mecidine ,Benha university
Abstract
Introduction: Intestinal mucositis is the most prevalent side effect of antineoplastic drug 5-flurouoracil (5-FU). Probiotics are live microorganisms that improve intestinal immune regulation. So, probiotics might have a beneficial effect to reduce the chemotherapy-induced enterotoxicity. Aim: This study was designed to assess mucositis induced by 5-FU and the possible protective effect of probiotics Methods: 50 healthy albino adult male rats were divided into five equivalent groups. Control group, Probiotic group was given 100 µL of suspension containing 1x107 ( CFU) of probiotic cocktail daily.5-FU group was received 50 mg/kg B.W. of 5-FU by intraperitoneal (IP) injection once daily for 5 consecutive days. Probiotic plus 5-FU group was given 50 mg/kg B.W. of 5-FU by IP injection for 5 consecutive days with probiotic cocktail once daily for 9 consecutive days. Recovery group was received 50 mg/kg body weight 5-FU by IP injection once daily for 5 consecutive days then left without medication till rats had been sacrificed on 9th day .The intestinal tissues have been processed for a light microscopic study.
Results: Rats of 5-FU group showed broadening, fusion and sloughing of villi into lumen, ulcerations, marked depletion of goblet cells, strong iNOS, marked caspase 3 and weak Ki-67 immunoreactions . Rats treated with 5-FU plus probiotic showed preservation of normal intestinal architecture with improvement of histological and immunohistological features of mucositis . Rats of recovery group showed decrease histological and immunohistochemical features of mucositis.
Conclusion: oral intake of the probiotic can protect against chemotherapy-causing intestinal mucositis in a rat model.