The possible protective role of oxytocin on hepatic ischemia and reperfusion injury via modulation of NADPH oxidase in adult male rats.

Document Type : Original Article

Authors

1 Physiology Department, Faculty of Medicine ,Benha University ,Benha ,Egypt

2 Physiology Department, Faculty of Medicine, Benha University, Benha, Egypt

Abstract

Background: Hepatic ischemia reperfusion injury (HIRI) is considered one of the most common causes of liver damage and dysfunction. oxytocin (OT), besides its classical functions, exhibits a potent antistress, anti-inflammatory, and antioxidant effects. Aim: This study was designed to evaluate the effect of pretreatment with OT on HIRI and to determine its possible protective mechanisms, focusing on the potential role of NADPH oxidase 2 (NOX2). Methods: 28 adult Wister albino male rats divided into 4 groups: group I (control group): received saline and subjected to surgery without ischemic procedure, group II (OT group): received OT and subjected to surgery without ischemic procedure, group III (HIR group): underwent hepatic ischemia reperfusion (HIR) procedure & group IV (HIR+ OT group): received OT and underwent HIR procedure. We assessed the effect of OT on serum liver enzymes, hepatic malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor alfa (TNFα), and NOX2. Results: HIR caused significant increases in serum liver enzymes, hepatic MDA, TNFα and NOX2 levels with a significant decrease in GSH level. Administration of OT caused a significant improvement in all previous parameters, these results were supported by histopathological examination. Conclusion: OT exerts hepatoprotective effect in HIR-induced liver injury even in part through NOX2.

Keywords

Benha Medical Journal
Volume 40, Special issue (Academic)
September and October 2023
Pages 35-47

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