Benha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Possible Beneficial Effect of Metformin Alone or in Combination with Methotrexate in Rheumatoid Arthritis Induced Rat Model1431548950510.21608/bmfj.2020.89505ENNasr MakarDepartment of clinical pharmacology, Benha faculty of medicine, Benha University, EgyptAbdel-Hamid ElhawaryDepartment of clinical pharmacology, Benha faculty of medicine, Benha University, EgyptHanan EmamDepartment of clinical pharmacology, Benha faculty of medicine, Benha University, EgyptNashwa Abo RiaDepartment of clinical pharmacology, Benha faculty of medicine, Benha University, EgyptEnas El-SayedShaabanassistant lecturer of pharmacology, clinical pharmacology department, faculty of medicine, Benha universityJournal Article20200515Background: Rheumatoid arthritis (RA) is a chronic, relapsing inflammatory and autoimmune multisystem illness that affects the joints. Methotrexate (MTX) is considered by many rheumatologists to be the most important and useful [DMARDs]. Metformin an antidiabetic medication has been reported to exert an anti-inflammatory effect. The rationale of this study is to test the effect of monotherapy with metformin alone or in comparison with methotrexate monotherapy on Adjuvant-induced arthritis (AIA). Aim of the study: The present study was designed to evaluate the effect of metformin alone and during basal treatment with methotrexate on rheumatoid factor, C-reactive protein, reduced glutathione, tumor necrosis factor- α, arthritis score and histopathological changes. Materials and methods: Rats were classified into: Group I: control normal group. Group II: was not treated (diseased group). Group III was treated with methotrexate (MTX). Group IV: was treated with metformin. Group V: was treated with combination of MTX with metformin. All treated groups received drugs for 4 weeks. Results: All groups showed significant improvement in all parameters and improvement of the histopathology of the joint. A significant reduction in the score was seen in all treated group at the end of 3rd and 4th weeks. Conclusion: All tested drugs alone or in combination showed improvement of parameters of RA. It is found that all combinations show more efficacy than each drug alone.https://bmfj.journals.ekb.eg/article_89505_c09b1d9783c825759656d3133c4d4dcb.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Role of Serum Amyloid A Protein in the Early Detection of Late Onset Sepsis in Neonate1551687393210.21608/bmfj.2020.73932ENHeba MohammedAbd Elkhalekpediatric, benha faculty of medicineNeeven AbedDepartment of pediatric, Benha faculty of medicine, Benha University, EgyptOmima Abdel HaieDepartment of pediatric, Benha faculty of medicine, Benha University, EgyptSeham GodaDepartment of Clinical and Chemical Pathology Faculty of Medicine, Benha University, EgyptJournal Article20200226<strong>Background: </strong>Late onset sepsis (LOS) is an important cause of serious illness and deaths among neonates. Diagnosis of neonatal sepsis remains a challenge owing to nonspecific early clinical signs and the non-availability of a reliable biomarker. Serum amyloid A (SAA), the precursor protein in inflammation-associated reactive amyloidosis, whose level in the blood increases up to 1000 fold in response to inflammation and it is also an acute phase reactant like PCT and CRP. Objectives: assessing the accuracy and rapidity of SAA in detection of LOS in neonates<strong>. Method</strong>: This is a case-control study which was carried out on neonates admitted in (NICU) of Benha University Hospital and Benha Teaching Hospital during the period (from June 2018 to November 2019). Group 1 (patients group): 45 neonates with neonatal sepsis, group 2 (control group): 40 healthy neonates age and sex matched. SAA was measured<strong>. </strong><strong>Results: </strong>mean value of SAA in septic group was 38.8 µg/ml compared to 1.26 µg/ml in control group , with statistically significant increase in patients than controls (<em>p</em><0.001). ROC curve was done to show the performance of SAA in the prediction of LOS. It was found that at a cut-off value of SAA <span style="text-decoration: underline;">></span>2.8 µg/ml, SAA had a sensitivity 86.7%, specificity 85%, PPV 86.7%, and NPV 85% for early diagnosis of LOS. At a cut-off value of SAA<span style="text-decoration: underline;">></span>45.2 µg/ml, SAA had a sensitivity 83.3%, specificity 69.7%, PPV 50%, and NPV 92% for the prediction of mortality among LOS patients. <strong>Conclusion: </strong>The high sensitivity, specificity, positive predictive value and negative predictive value of SAA protein could help the clinicians for early diagnosis of LOS.https://bmfj.journals.ekb.eg/article_73932_8e83e1e3f3a34d33318a0fa36181affc.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Effect of Proton Pump Inhibitors, Vitamin E and their co- administration on heart function and Oxidative Changes in Isoprenaline Induced Myocardial Infarction in Adult Male Albino Rats1691837740410.21608/bmfj.2020.77404ENAbeer AhmedShomandepartment of physiology, faculty of medicine, Benha universityAhmed Badwydepartment of physiology 6th October Univerisity, Egypt.Mohamed ElhammadyDepartment of physiology, Benha faculty of medicine, Benha University, EgyptYara Eldesokidepartment of physiology 6th October Univerisity, EgyptJournal Article20200315<strong>Background: </strong>Myocardial infarction (MI) continues to be associated with high morbidity and mortality worldwide. Proton Pump inhibitors (PPIs) exhibit cardioprotective effects by several mechanisms. Vitamin E possesses antioxidant properties. <strong>Aim</strong>: The present study has been designed to evaluate the combined cardioprotective effect of PPIs and vitamin E on biochemical and histopathological alteration in isoprenaline (ISO) induced myocardial infarction in rats. <strong>Methods</strong>: 30 adult Wistar albino male rats divided into 5 groups as follow: Group I: Control fed a standard diet.Group II: rats were undergoing MI by ISO. Group III: Rats were pretreated pantoprazole (1.3 mg/kg, i.o) daily for 3 weeks. Group IV: Rats were pretreated with vitamin E (100 IU/kg i.o) daily for 3 weeks. Group V: Rats were given both pantoprazole (1.3 mg/kg, i.o) and vitamin E (100 IU/kg bw, <em>i.p)</em> daily. On 22<sup>th</sup>and 23<sup>th</sup> day, rats in groups (II-V) were injection <em>i.p</em> with ISO (100mg/kg bw for successive two days). <strong>Results</strong>: ISO induced infarction in rats resulted in a significant elevation in ST segment height, T – wave voltage, Heart rate (HR), serum lactate dehydrogenase (LDH), malondialdehyde (MDA) and nitric oxide metabolites (NO), whereas it caused a significant (P < 0.05) decrease in systolic blood pressure(SBP) and serum superoxide dismutase (SOD) when compared with the control group. Moreover, interventions with PPIs supplementation and/or vitamin E significantly reversed the aforementioned parameters with better impact of their combination. <strong>Conclusion</strong>: PPIs combined with vitamin E exert potential protective effect against MI through lowering calcium overload and enhancement of antioxidant activity.https://bmfj.journals.ekb.eg/article_77404_f867a0595a9b96030291847efa794f79.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Predictive and Prognostic Relevance of p53 in Patients With Serous Epithelial Ovarian Cancer1841927740610.21608/bmfj.2020.77406ENHind AbdelhamidElgenediClinical oncology, Mansoura university, Dakahleyia, EgyptInas AbdelhalimDepartment of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Mansoura University EgyptMahfouz EitaDepartment of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Mansoura University EgyptMaiy ElshahatDepartment of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Mansoura University EgyptMohammed Arafadepartment of Pathology, Faculty of Medicine, Mansoura University, EgyptJournal Article20200315<strong>Background: </strong>Ovarian cancer is a common malignant gynecological tumor that is difficult to diagnose early, progresses rapidly, and causes high mortality. Aims of our study: to assess the relationship of P53 with other clinico-pathological parameters and the effect of P53 on patients’ out-come.<br /> <strong>Subjects and methods</strong>: This study was conducted at Clinical Oncology and Nuclear Medicine Department with Pathology Department, Faculty of Medicine, Mansoura University. The study was carried out on 50 patients with serous epithelial ovarian cancer presented to receive adjuvant treatments following a primary surgery and were followed from the first day after surgery, follow-up started on January, 2012 till February, 2016. P53 expression was assessed immunohistochemically on formalin-fixed paraffin-embedded tissues, and Secondary red Envision system. Patients were given adjuvant treatment(s) according to according to NCCN guidelines. The primary endpoint of the study was loco regional recurrence, and distant metastasis. At the end of the follow-up period, the patient clinico-pathological data and patient outcome were collected.<br /> <strong>Results: </strong>we found that p53 negative tumors have a better OS & DFS at 3 year than with P53 positive tumors, but not reached statistically significant differences ( p value = 0.98 & 0.48 respectively).<br /> <strong>Conclusion: </strong>Our results showed no statically significant difference between p53 expression. OS & DFS, need to be evaluated in other study including large number of patients before using it as a marker for the outcome in these tumors.https://bmfj.journals.ekb.eg/article_77406_bb4c3e4e3b95f8b67fe02ce516510d7f.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Histological and immunohistochemical study on the possible therapeutic role of stem cells and curcumin in cyclophosphamide-induced cardiotoxicity in adult male albino rat1932068598410.21608/bmfj.2020.85984ENAzza IbrahimHelalHistology department,faculty of medicine,kafr sheikh universityOmayma HelalDepartment of histology and cell biology, Benha faculty of medicineHala Metwalydepartment of clinical pathology, Cairo universityMaha Abo Gaziadepartment of Histology, Benha faculty of medicine, kafr sheikh universityOla MohamedDepartment of histology and cell biology, Benha faculty of medicineJournal Article20200428<strong>Introduction: </strong>Cyclophosphamide (CP) is used as a chemotherapeutic and an immunosuppressive agent. CP is known to cause multiple organ toxicity; the most obvious is cardiotoxicity.<strong> Aim: </strong>Possibile therapeutic effect on bone marrow -derived mesenchymal stem cells (BM-MSCs) in ameliorating CP- induced cardiotoxicity compared with Curcumin in albino rats.<strong> Methods: </strong>Fifty male albino rats were divided into five groups: group I received intraperitoneal injection of sterile water; group II received oral curcumin (200 mg/kg); group III received only one intraperitoneal injection of CP (200 mg/kg) and were sacrificed on day 40; group IV received the same treatment as group III followed by oral administration of curcumin from day 10 and were sacrificed on day 40; group V received the same treatment as group III followed by a single BM- MSCs injection intraperitoneally at a dose of 1<em>×</em>10<sup>6</sup> cells/ rat on day 10 and were sacrificed on day 40. <strong>Results:</strong> Histological structure of the cardiac muscle by light and electron microscopic examination revealed marked structural changes in rats treated with CP alone. Improvement in BM-MSCs group more than curcumin treated group was observed. Immunohistochemical staining of the cardiac muscle showed strong positive immunoreactivity for caspase-3 in group III compared to the control and other groups. Also, BM-MSCs extensively reduced the amount of collagen fibers compared with other groups. <strong>Conclusion:</strong> The use of curcumin has a limited beneficial effect on the protection of cardiac muscle against CP toxicity compared with stem cells.https://bmfj.journals.ekb.eg/article_85984_773b67c79bed97395312fba1f02e4c56.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501The Significance of Aldehyde Dehydrogenase 1A1 Expression in Benign Prostatic Hyperplasia, Prostatic Intraepithelial Neoplasia and Prostatic Adenocarcinoma2072198200310.21608/bmfj.2020.82003ENSara GamalMasoudpathology department, faculty of medicine, benha university, benha, elqaluobia, egyptMagda BakrDepartment of pathology, faculty of medicine, Benha university, Benha, EgyptNashwa EmaraDepartment of pathology, faculty of medicine, Benha university, Benha, EgyptSarah NasifDepartment of pathology, faculty of medicine, Benha university, Benha, EgyptJournal Article20200412<strong>Background: </strong>Prostatic carcinoma contains a small population of cancer stem cells (CSCs) that promote tumor growth, maintenance, and progression. CSC markers could provide a prognostic tool for human malignancies. Therefore, CSCs may have a role in prostatic carcinoma. This study aimed to evaluate the expression patterns and significance of stem cell marker aldehyde dehydrogenase 1A1 (ALDH1A1) in prostatic adenocarcinoma. <strong>Methods</strong>: This is a retrospective study performed upon 60 cases grouped as; 10 cases (16.7%) of benign prostatic hyperplasia (BPH), 6 cases (10%) of high-grade prostatic intraepithelial neoplasia (HGPIN) and 44 cases (73.3%) of prostatic adenocarcinoma (PCa) of different Gleason scores. Immunohistochemistry was applied on formalin-fixed, paraffin-embedded blocks using ALDH1A1. The relation between ALDH1A1 expression and clinicopathologic parameters was assessed. <strong>Results: </strong>Low expression of ALDH1A1 was seen in all cases (100%) of BPH, 5/6 (83.3%) of HGPIN and 12/44 (27.3%) of PCa, while high expression was in 1/6 case (16.7%) of HGPIN and 32/44 (72.2%) of PCa cases. There were significant relations between the expression levels of ALDH1A1 in PCa compared with HGPIN and BPH cases (P<0.01). ALDH1A1 expression showed highly significant associations with pre-operative serum PSA level, Gleason score, Gleason Grade Group (P<0.01) and was significantly associated with perineural invasion and tumor stage (P<0.05). No significant relations were found between ALDH1A1 expression and patients` age, capsular invasion, lymphovascular invasion, depth of invasion (pT) or lymph node metastasis. <strong>Conclusion: </strong>ALDH1A1 is a prostate CSC marker, could be involved in tumorigenesis and progression of prostatic adenocarcinomahttps://bmfj.journals.ekb.eg/article_82003_386edfe4eb1229d39c1600eb38f22c1b.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Serum Lactate/Albumin Ratio as a Predictor Of Morbidity and Mortality In Patients with Severe Sepsis and Septic Shock2202288620510.21608/bmfj.2020.86205ENEssam MakramDepartment of Anaesthesia and Intensive Care, Benha Faculty of Medicine, Benha UniversityMohamed Ibrahimdepartment of nephrology and Internal medicine , Faculty of Medicine – Benha University,Ahmed Abdel RahmanDepartment of Anaesthesia and Intensive Care, Benha Faculty of Medicine, Benha UniversityKarim AborayaDepartment of Anaesthesia and Intensive Care, Benha Faculty of Medicine, Benha UniversityJournal Article20200501<strong>Background:</strong> Severe sepsis is common and frequently fatal and represents a significant health care burden. Sepsis is a syndrome of physiologic, pathologic, and biochemical abnormalities induced by infection. <strong>Aim of the work:</strong> This study examines the ability of serum lactate/albumin ratio to predict outcome regarding organ dysfunction and mortality in severe sepsis and septic shock.<strong> Materials and Methods</strong>: It is a prospective observational study conducted on 50 patients with severe sepsis and septic shock according to the (SCCM/ESICM/ACCP/ATS/SIS) International Sepsis Definitions Conference 2001 and fifty healthy age matched individuals as a control group.<strong> Results:</strong> Lactate/albumin ratio was higher on day 0 and 1 in the study group than in control group (<em>p</em> value <0.001). Also, it was significantly higher in patients that needed mechanical ventilation than patients who didn’t (<em>p</em> value p valuep value<0.001).<strong>Conclusion: </strong>Lactate/albumin ratio is a reliable biomarker for prediction of outcome as regards organ dysfunction and mortality in patients with severe sepsis and septic shock.https://bmfj.journals.ekb.eg/article_86205_133c5ce662d8c13accda90ed376833a8.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Effect of exenatide, metformin and folic acid on experimentally induced metabolic–cognitive syndrome in rats2292458639610.21608/bmfj.2020.86396ENAhmed BahrizDepartment of clinical pharmacology, Benha faculty of medicine, Banha University, EgyptMohamed MansourDepartment of clinical pharmacology, Benha faculty of medicine, Banha University, EgyptAmany IbrahimDepartment of clinical pharmacology, Benha faculty of medicine, Banha University, EgyptDoaa KhalilDepartment of clinical pharmacology, Benha faculty of medicine, Banha University, EgyptHeba El-NouryDepartment of clinical pharmacology, Benha faculty of medicine, Banha University, EgyptJournal Article20200502<strong>Background: </strong>Metabolic syndrome (MetS) and its relationship with cognitive impairment has been the subject of extensive research. <strong>Purpose</strong>: This study was designed to determine the effect of MetS on cognitive function, and the possibility of modulating this effect by exenatide, metformin and folic acid. <strong>Materials and Methods:</strong> 30 adult male albino rats were divided in 5 groups. <strong>Group (I):</strong> received a standard rat chow, <strong>group (II): </strong>none treated rats with MetS fed with 60% fructose added to the standard rat chow, <strong>group (III): rats with </strong>MetS treated with exenatide, <strong>group (IV): rats with </strong>MetS treated with metformin,<strong> group (V): </strong> rats with MetS treated with folic acid. At the end of the experiment, fasting blood glucose, fasting plasma insulin, HOMA-IR index, serum triglyceride, HDL-C, dopamine and BDNF levels in brain tissue were measured and cognitive performance was assessed by Morris water maze (MWM) test. <strong>Results: </strong>rats with MetS showed increased levels of fasting blood glucose, fasting plasma insulin, HOMA-IR index, arterial blood pressure, serum triglycerides, decreased HDL-C, dopamine and BDNF and showed memory impairment in MWM test. All treated groups resulted in decrease in fasting blood glucose, fasting plasma insulin, HOMA-IR index, arterial blood pressure, and serum triglycerides and increase in HDL-C, dopamine and BDNF as well as improvement in MWM test. <strong>Conclusion: </strong>MetS was associated with cognitive impairment. Exenatide, metformin and folic acid improved cognitive function in addition to improvement of metabolic parameters.https://bmfj.journals.ekb.eg/article_86396_f593f6cf712ad1f80e9d5990427fc2c2.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Hypofractionated Whole Breast Radiotherapy with Simultaneous Integrated Boost Following Breast Conservative Surgery for Early Breast Cancer2592707964610.21608/bmfj.2020.79646ENAhmed RamadanEldesokyClinical Oncology and Nuclear Medicine Department, Mansoura University, Mansoura, EgyptAhmed ElshahatDepartment of Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine, Mansoura University EgyptMona EskanderDepartment of Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine, Mansoura University EgyptMona FoudaDepartment of Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine, Mansoura University EgyptJournal Article20200330Background: Hypofractionated whole breast radiotherapy (HFWBRT) showed comparable efficacy and safety to conventional fractionated radiotherapy. Dose and fractionation of the tumor bed boost to be integrated during HF-WBRT schedules is still to be determined. Aim: to investigate the clinical feasibility of HF-WBRT with simultaneous integrated boost (SIB), in early breast cancer patients who underwent breast conserving surgery (BCS). Methods: This single arm prospective study included 40 female patients with pathologically proven stage I-II breast cancer following BCS with high risk factors. Patients received 3D conformal radiotherapy (3DCRT) with field in field (FIF) technique. The whole breast received a dose of 40 gray (Gy) over 15 fractions for 3 weeks with an additional SIB dose of 8 Gy over 15 fractions to be give daily during WBRT. Radiation toxicities were graded using the common terminology criteria for adverse events (NCI-CTCAE) scale version 4.03. Cosmetic outcome was assessed using Harvard cosmetic score. Kaplan Meier method was used to estimate the 3- year disease free (DFS) and overall survival (OS). Results: The median age at presentation was 48 years (range 33-68). No reported grade 3 or 4 toxicities. Grade 1 and 2 radiation dermatitis affected 80% of the patients, breast pain was observed in 62.5% of the patients while 25% had radiation pneumonitis. Most of the patients (95%) had excellent and good cosmetic outcomes. At 3 years the estimated DFS was 95% and OS was 97.5 %. Conclusion: WBRT-SIB using 3DCRT with FIF technique is clinically feasible for early stage breast cancer patients.https://bmfj.journals.ekb.eg/article_79646_dc3aac906f8313426b013f063eb39968.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Laparoscopic Appendectomy Versus Open Appendectomy in Young Female Patients2712848586810.21608/bmfj.2020.85868ENAbdelmonem Aligeneral surgery faculty of medicine benha university0000-0002-6572-8006Hassan Alsouenigeneral surgery faculty of medicine benha universityMohammed, Mohammed,general surgery faculty of medicine benha universityAhmed Nawargeneral surgery faculty of medicine, Benha universityJournal Article20200428<strong>Background: </strong>Laparoscopic appendectomy combines the advantages of diagnosis & treatment in single procedure with least morbidity. In addition, whole abdomen can be visualized to rule out other coexisting pathology. Patients are likely to have less postoperative pain and be discharged from hospital and return to activities and routine work sooner than those who have undergone open appendectomy. <strong>Aim of the work</strong>: The purpose of the work was to describe our experience in comparative evaluation of modified laparoscopic versus open appendectomy for the treatment of acute appendicitis as regard surgical techniques, operative time and hospital stay in young females. <strong>Methodology:</strong> Our retrospective study has been conducted in General Surgery Department of Benha University Hospital from Oct 2017 to March 2018 on 40 patients. <strong>Results:</strong> Our results showed that laparoscopic appendectomy has many advantages such as: shorter hospital stay, decreased need for postoperative analgesia, earlier return to work and lower rate of wound infection. <strong>Conclusion:</strong> Laparoscopic appendectomy is safe and effective, less postoperative pain, early return to normal work and better cosmetic scar than open appendectomy.https://bmfj.journals.ekb.eg/article_85868_98067604aa9ee65d8e9f93d0440f5124.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Effectiveness Of Mesotherapy In Treatment Of Fibromyalgia Tender Points2852968951210.21608/bmfj.2020.89512ENRefaat EltanawyDepartment of Rheumatology, Rehabilitation and Physical Medicine, Benha faculty of medicine, Benha UniversitySahar GanebDepartment of Rheumatology, Rehabilitation and Physical Medicine, Benha faculty of medicine, Benha UniversityRasha FawzyDepartment of Rheumatology, Rehabilitation and Physical Medicine, Benha faculty of medicine, Benha UniversitySamia GhorabDepartment of Rheumatology, Rehabilitation and Physical Medicine, Benha faculty of medicine, Benha UniversityJournal Article20200515Background: Fibromyalgia is the second most common disease encountered in rheumatology practice Aim: This study designed to assess the potential effect of mesotherapy in treatment of tender points in primary fibromyalgia (PFM) patients. Methods: Thirty PFM patients who fulfilled the American Colleague of Rheumatology 1990 criteria, joined this study. They were divided into 2 groups randomly according to the treatment program received; Injection mesotherapy group (Group I: No=15 patients) who received a mixture of 2 ml of Ketoprofen (100mg/2ml) added to 3 ml of Saline Solution, once cession per week for a total of 6 successive weeks and other 15 patients (Group II) who received Ketoprofen 100 mg Extended- Release capsules once daily for 2 successive weeks which represent the control group. Patients‟ pain severity, quality of life and depression were assessed using visual analogue scale (VAS), fibromyalgia impact questionnaire (FIQ) and the Hamilton Rating Scale (HDRS) respectively before treatment and after the 3rd & the 6th cession then data were recorded and compared. Results: We found significant improvements (p<0.001) tender point‟s number, VAS, FIQ and HDRS in the injection mesotherapy group compared to the control group with no major adverse effects were described. Conclusion: This study supports the hypothesis that injection mesotherapy could offer a great therapeutic advantage in treatment of fibromyalgia tender points especially in the presence of associated comorbidities where there is a high risk of drug interaction, polypharmacy, or when conventional (oral or parenteral) NSAIDs use is contraindicated.https://bmfj.journals.ekb.eg/article_89512_7d5e3674b4a113f3af211507e8a8650b.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Epidemiological Study of Vitiligo in Damanhour Teaching Hospital2973047965910.21608/bmfj.2020.79659ENIhab AbdallahDepartment of Dermatology and Andrology, Faculty of Medicine, Benha University
Egypt,Osama HusseinDepartment of Dermatology and Andrology, Faculty of Medicine, Benha University
Egypt,Amany AbdelmagidaDepartment of Dermatology and Andrology, Faculty of Medicine, Benha University
Egypt,Journal Article20200330<strong>Background: </strong>Vitiligo is the most common skin depigmenting disorder resulting from a selective loss of epidermal melanocytes, and affects around 0.5% of the world population. Both sexes are affected, and there are no apparent differences in rates of occurrence according to phototype or race.<strong> Aims: </strong>This study was doneto evaluate the epidemiology of vitiligo in Damanhour teaching hospital and to assess the clinical presentations of vitiligo.<strong> Patients and methods : </strong>This is a cross-sectional study on patients of vitiligo among cases who attended the dermatology and andrology outpatient clinic in Damanhour teaching hospital ,El-Behira ,Egypt .A predesigned dermatological sheet was used for data collection from the first 10,000 patients during a period of six months. Patients were clinically examined to determine type of skin diseases they have and those with vitiligo were selected.<strong> Results</strong> : Out of 10,000 studied patients, there were 86 cases of vitiligo with a prevalence of 0.86%. The results revealed a female predominance. There was a predominance of patients from rural areas. The most common associated disorder was thyroid disease.<strong>Conclusion: </strong>the prevalence of vitilgo in Damanhour teaching hospital is 0.86% and it is slightly more common among females and in rural areas. The most common exacerbating factor is stress.https://bmfj.journals.ekb.eg/article_79659_d8203fa6de95189d419ad027494677c6.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Significance of Insluin-Like Growth Factor II mRNA Binding Protein 3 (IMP3) Expression In Selected Thyroid Lesions3053188573910.21608/bmfj.2020.85739ENTaghreed Abd ElAzizDepartment of pathology, Benha University, EgyptRanih AmerDepartment of pathology, Benha University, EgyptOmnia BassyoniDepartment of pathology, Benha University, EgyptHanan AhmedDepartment of pathology, Benha University, EgyptJournal Article20200426<strong>Background: </strong>Differentiation of benign from malignant follicular thyroid lesions remains difficult and the ability of molecular markers to differentiate between them still unclear. The aim of this study was to evaluate the usefulness of IMP3 expression to distinguish benign from malignant thyroid lesions. <strong>Methods</strong>: This is a retrospective study upon selected 57 thyroid lesions designated as; 7 cases of Hashimoto thyroiditis (HT), 10 cases of hyperplastic nodules (HN), 15 cases of follicular thyroid adenoma (FTA), 13 cases of conventional papillary thyroid carcinoma (PTC), 6 cases of follicular variant of papillary thyroid carcinoma (FV-PTC), and 6 cases of follicular thyroid carcinoma (FTC). Immunohistochemistry was applied on formalin-fixed, paraffin-embeded tissue blocks using IMP3. clinicopathological data were reviewed from patients' pathological reports and correlated with IMP3 expression. <strong>Results: </strong>IMP3 positivity was seen in1/7 cases (14.3%) of HT, 2/10 cases (20%) of HN, 4/15 cases (26.7%) of FTA, 12/13 cases of PTC, and in all (100%) FV-PTC & FTC cases. IMP3 staining was significantly increased from normal thyroid tissue up to malignant tumors (P<0.01). IMP3 showed 96% sensitivity and 78.1% specificity for malignant tumors. IMP3 expression was positively correlated with grade and tumor size in malignant cases (P<0.05). No significant correlation was found in IMP3 expression with patient age, sex, capsular invasion, lymphatic/vascular invasion, lymph node metastasis, distant metastasis and TNM stage. <strong>Conclusions: </strong>IMP3 is a potential diagnostic marker for thyroid cancer and can be a promising marker for distinguishing benign from malignant follicular patterned thyroid lesions.https://bmfj.journals.ekb.eg/article_85739_c78804e821de5fcecc4fc6019b7376c7.pdfBenha University, Faculty of MedicineBenha Medical Journal1110-208X37120200501Lipid Profile in Relation to Severity of Liver Diseases3193258951310.21608/bmfj.2020.89513ENAhmed GomaDepartment of Internal Medicine, Faculty of Medicine, Zagazig University, EgyptMahmoud SharafeddinDepartment of Internal Medicine, Faculty of Medicine, Zagazig University, EgyptAmany AbdAllahDepartment of Family Medicine, Faculty of Medicine, Zagazig UniversityJournal Article20200515Background: Liver cirrhosis is a life-threatening condition. It is very prevalent in Egypt. Lipids are stored and metabolized in the liver. Liver cirrhosis has an essential influence on the lipid profile. Objectives: Evaluation of the lipid profile via assessing triglyceride (TG), total cholesterol, very low-density lipoprotein, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) in cirrhotic patients and define its relationship to Child score. Patients and methods: This cross-sectional study was carried out in the Internal Medicine Department outpatient clinic, Zagazig University Hospital, Egypt. Ninety patients with liver cirrhosis were enrolled in the study. Fasting lipid profile was done. Comparison of the lipid profile in different Child classes in cirrhotic was done. Results: Total cholesterol, HDL, LDL, VLDL, and TG were compared in different childturcotte-pugh (CTP) classes. The data was analyzed on SPSS 21. Results showed significantly low levels of total cholesterol, HDL, LDL as the CTP class advances and also lower levels as compared with the control group. However, a statistically non-significant decrease in TG was observed. Conclusion: Total cholesterol, HDL, and LDL levels are significantly reduced in patients who develop liver cirrhosis and levels further decrease as the CTP classadvances. Keywords: CTP score, lipid profile,https://bmfj.journals.ekb.eg/article_89513_5e8994fe257ba12abc243c0791740cd4.pdf